Table 2.
Analysis of the CDSs deposited on the XACarray based on the hybridization signal during the experimental validation of the platform.
| Type of clones | Physical analysis (Fixed PCR products) | Annotationc | Hybridization signal | ||||
|---|---|---|---|---|---|---|---|
| Clones n° (%) |
AISa (bp) |
C/Ib (%) |
Function n° (%) |
Hypothetical n° (%) |
Function n° (%) |
Hypothetical n° (%) |
|
| I | 1,184 (57.6) | 1,487 | 56.8 | 631 (53.2) | 553 (46.8) | 574 (90.9) | 485 (87.7) |
| II | 292 (63.1) | 1,121 | 100 | 235 (80.5) | 57 (19.5) | 215 (91.5) | 52 (91.2) |
| III | 490 (59.3) | 1,211 | 69.9 | 343 (70.0) | 147 (30.0) | 323 (94.2) | 123 (83.7) |
| IV | 673 (59.9) | 1,209 | 70.5 | 466 (69.2) | 207 (30.8) | 436 (93.6) | 184 (88.9) |
| Total | 2,639 (59.7) | 1,324 | 82.1 | 1,675 (63.5) | 964 (36.5) | 1,521 (90.8) | 844 (87.6) |
| 2,365 (89.6) | |||||||
a AIS (average of insert size).
b C/I (average of overlapping size CDS/Insert)
c Annotation based on Silva et al 2002 [6].
Note that the number of clones that were amplified and positively annotated represent only 59.7% of the total clones initially selected (4,421), and that 2,365 of individual CDSs presented a positive hybridization signal, which represent 52.7% of the total annotated CDSs in the genome and 89.6% of the fixed products.