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Alignment of nucleotide sequences of the S-gene product. Representative sequence of genotypes A (X75669) and D (X65259) are shown at the bottom of the raw. Inherent recognition sites of Nla IV in genotype D are shaded. T-to-C nucleotide substitution occurred at nt 291 (box) in KWT-43 (local HBV isolate). These substitutions resulted in generation of a new recognition site of Nla IV (motif: GGNNCC).
