Table 3.
Sequence alignment of the loop domain linking β2 to α2 of YiiP and representative members of the cation diffusion facilitator family.
| CDF homologs | Amino acid sequence | Residue number |
|---|---|---|
| ◀----β2---▶ ◀-L3-▶ ◀----- α2 -------- | ||
| YiiP | PTRFIQIHLE MEDSLP LVQAHMVADQVEQAI | 286 |
| CzrB | PRSFLEFHLV VRGDTP VEEAHRLCDELERAL | 269 |
| ZitB | EKPVMTLHVQ VI---P PHDHDALLDQIQHYL | 275 |
| CzcD | GKASLTVHVV NDTA-- VNPEMEVLPELKQML | 273 |
| msZnT-8 | NQVILSVHVA TA---A SQDSQSVRTGIAQAL | 337 |
| hmZnT-2 | AQPVLSVHIA IAQN-- -TDAQAVLKTASSRL | 293 |
| hmZnT-3 | TYHVASAHLA IDST-- -ADPEAVLAEASSRL | 357 |
| hmZnT-8 | NQVILSAHVA TA---A SRDSQVVRREIAKAL | 339 |
The human ZnT-8 residue R325 is in boldface. YiiP and ZitB are bacterial CDF homologs from Escherichia coli, CzcD from Ralstonia metallidurans, CzrB from Thermus thermophilus, msZnT-8 from Mus musculus (mouse), and ZnT-2, ZnT-3, and ZnT-8 from Homo Sapiens.