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. 2010 May 29;16:945–953.

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Copyright © 2010 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Isolation and characterization of side population (SP) cells from the mouse lens epithelium. Fluorescence-activated cell sorting (FACS) analysis of mouse lens epithelial cells stained with Hoechst 33342 alone (A) or in the presence of verapamil (B). The P1 region shows the gated region identified SP cells. The P2 region is designated as non-SP cells. SP cells (C) and non-SP cells (D) were observed by phase contrast microscopy. The scale bar represents 10 µm. (E) Forward scatter characteristics (FSC) of SP cells (solid area) and non-SP cells (open area) were analyzed by FACS.