Table 1.
Probe Technology | MiRBase version | One or two-color analysis | Number of miRNAs considered | Correlation | Standard Error | 95% Confidence intervals | |
---|---|---|---|---|---|---|---|
Agilent Mouse miRNA (P/NG4472A) | 60 mer probes with hairpin loop structure and 5'G residue complement to the C residue added to the microRNA during labelling | MirBase 12 | One | 122 | 0.995 | 0.0014 | 0.991-0.997 |
54 | 0.995 | 0.0035 | 0.984-0.997 | ||||
LC Science Mouse miRNA Microarray MRA-1002 | μParafloTM Technology uses proprietary in-situ oligo synthesis, microfluidic reaction cells, and novel photochemistry coupled with photolithography; full content flexibility | MirBase 12 | One | 131 | 0.978 | 0.0068 | 0.961-0.987 |
54 | 0.981 | 0.0105 | 0.952-0.992 | ||||
Exiqon miRCURY LNA™ microRNA Array kit | LNA Locked Nucleic Acids are conformationally locked into a favourable confirmation for miRNA binding | MirBase 11 | Two | 131 | 0.914 | 0.0189 | 0.870-0.944 |
54 | 0.913 | 0.0258 | 0.848-0.948 | ||||
One | 125 | 0.874 | 0.0330 | 0.798-0.925 | |||
54 | 0.802 | 0.0723 | 0.631-0.910 | ||||
Invitrogen NCode Multi-Species miRNA Microarray Probe Set V2 | 34-44 nucleotide probes designed as dimers of sequences complementary to mature miRNAs, trimmed to reduce melt temperature variability between probes. | MirBase 9 | Two | 118 | 0.706 | 0.0544 | 0.586-0.798 |
54 | 0.816 | 0.0532 | 0.686-0.892 | ||||
One | 124 | 0.853 | 0.0338 | 0.775-0.907 | |||
54 | 0.850 | 0.0553 | 0.716-0.928 |
Spearman correlations and the 95% confidence intervals are presented for the miRNAs that were present within a platform from the sequence-matched list, as well as the 54 miRNAs that gave detectable signals on all four platforms.