Figure 3. Homozygous Sdc^{BG0277 4} flies have lower expression levels of dilps, fecundity, and metabolic activity.

(A–B) Gene expression levels were measured by RT-qPCR using mRNA extracted from heads (dilps) and whole-body (Akh) of female (panel A) and male (panel B) flies (n = 6). All genes were normalized to rp49. (C) Values represent the average number of eggs laid by female flies over a five day period per n = 10 independent replicates. (D) CO₂ production measured by indirect calorimetry. No differences were observed between male and female flies and the values represent VCO₂ least-square means of n = 20 independent replicates. (E) Waking activity was measured counting the number of times a given fly crosses an infrared beam during a one-minute interval. Values represent the average number of activity counts per waking minute of two independent replicates of 16 flies. In all panels, error bars represent SEM. *P≤0.05, ** P≤0.01, *** P≤0.001 compared to control.