Figure 4. Homozygous Sdc^BG02774 flies have lower expression levels of spargel and reduced mitochondrial function.

(A–B) Despite reduced levels of DILPs, homozygous Sdc^BG02774 flies do not show increased levels of Thor, but display lower levels of spargel mRNA than controls. Gene expression levels were measured by RT-qPCR using mRNA extracted from whole body of Sdc^BG02774 and CS (B) flies. Transcript levels of Thor and spargel were normalized to rp49. Values represent average of 17 (panel A) and 12 (panel B) independent replicates. (C) O₂ consumption by whole-fly isolated mitochondria was measured in the presence of saturating amounts of NAD⁺-linked respiratory substrates (pyruvate plus proline) and ADP (state 3 respiration rate). Values represent average of 20 independent replicates. In all panels, pooled male and female data was used for the analysis. Error bars represent SEM. ** P≤0.01, *** P≤0.001 compared to control.