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CYK48-DBL6ε domain and antibodies observed after purification on 10% acrylamide gel (Invitrogen) stained with Coomassie blue. Reduced CYK48-DBL6ε domain (lane 1) after two purification steps and non-reduced or reduced antibody from protein G purification (respectively lanes 2 and 3). Molecular weight on left lane (Biorad).
