Figure 6. Keratinocyte-specific TAK1 deletion causes a transition from anagen to dystrophic catagen in adolescent mice.

(A) Schedule of tamoxifen application. The hair cycle was synchronized to anagen phase by wax depilation. At 7 days after depilation, tamoxifen was applied for 5 days. (B) Clinical appearance of the mice 2 weeks after depilation. (C) Histological analysis of the mice 2 weeks after depilation. (D) Higher magnification of the tamoxifen-treated Map3k7 ^fl/flK14-Cre-ER^T2 mice in (C). Scale bar, 100 µm. (E) Dystrophic catagen was defined according to a previous report [28]. Then, the rate (%) of a certain hair follicle stage per total hair follicles was calculated. Quantitative analyses confirmed that most of the hair follicles in tamoxifen-treated Map3k7 ^fl/flK14-Cre-ER^T2 mice were in late dystrophic catagen, while those of controls were in anagen.