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. 2010 May 27;9:124. doi: 10.1186/1476-4598-9-124

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Copyright ©2010 Kim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of HOXB13 on p21-dependent E2F1-mediated transactivation: A, LNCaP cells were grown under hormone-deprived conditions for 3 days and transfected with pE2F1(3X)-luc, pFLAG-HOXB13, and pCDNA-E2F1. B, Both HCT116 (p21+/+) and HCT116 (p21-/-) cells were transfected with pE2F1-luc and an increased amount of pFLAG-HOXB13. C, HCT116 (p21+/+) cells were transfected with pE2F1-luc, pFLAG-HOXB13, and an increased amount of pFLAG-p21. Corresponding control vectors were used to match each DNA. After 48 hours, the cells were evaluated for luciferase assays. Each bar represents the mean ± S.D. D, S4 cells were treated with Dox to induce HOXB13 followed by Western blot and RT-PCR analyses.