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. 2010 May 14;8:47. doi: 10.1186/1477-7827-8-47

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Copyright ©2010 Liu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Localization and expression of Hsp27 in mouse oocytes from GV to MII cultured in vitro. (A) Immunofluorescence staining of Hsp27 and chromosomes in maturing oocytes. Oocytes culturing for 0 h (GV), 3 h (GVBD), 8 h (MI) and 14 h (MII) in vitro were stained with specific Hsp27 antibody (green) and propidium iodide (red). (B) Real time RT-PCR analysis of Hsp27 mRNA in maturing oocytes. Oocytes were cultured in vitro for 0, 3, 8 and 14 h. A progressive increase was observed with the maturation of oocytes. (C) Western blot analysis of Hsp27 expression in maturing mouse oocytes. Experiments were repeated at least three times. Bars = 20 μm. *P < 0.05, **P < 0.01 vs. control.