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Oocyte classification by Annexin-V staining. (A) necrotic oocytes. Discontinuous green signal was originated from the remnant portions of the membrane. (B) Oocyte Annexin-V negative: no signal in the cytoplasmic membrane. (C) Annexin-V positive (early stage of apoptosis): a clear green signal is observed in the oocyte membrane. Bars = 10 μm.
