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. 2010 May 14;8:47. doi: 10.1186/1477-7827-8-47

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Copyright ©2010 Liu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of Hsp27 downregulation in GV oocytes on the expression of some key apoptotic proteins and oocyte-derived growth factors. (A) Real time RT-PCR analysis in mouse oocytes after microinjecting Hsp27 antibody. Oocytes were cultured for 18-24 h. (B) Real time RT-PCR analysis in oocytes after infection with Ad-shHsp27. (C-F) Western blot analysis of key apoptotic factors in oocytes after infection with Ad-shHsp27. Measurements were plotted as the mean of at least three biological replicates ± SD. Changes are labeled as significant (*) if P < 0.05.