Table 1.
Primer sequences used for quantitative real-time PCR reactions.
| Primer name | Genbank accession number | Primer sequence | Location | Product size (bp) |
|---|---|---|---|---|
| Hsp27 | NM_013560 | F: 5'-GCCGCACCAGCCTTCAGC-3' | 357-374 | 147 |
| R: 5'-CACGCCTTCCTTGGTCTTCACT-3' | 482-503 | |||
| Caspase 3 | NM_009810 | F: 5'-ATGGGAGCAAGTCAGTGGAC-3' | 128-147 | 137 |
| R: 5'-CGTACCAGAGCGAGATGACA-3' | 245-264 | |||
| Caspase 8 | NM_001080126 | F: 5'-TGAAGGACAGAAAAGGAACAGA-3' | 996-1017 | 191 |
| R: 5'-CTTGTCACCGTGGGATAGGATA-3' | 1165-1186 | |||
| Caspase 9 | NM_015733 | F: 5'-GGCGGAGCTCATGATGTCTGTG-3' | 757-778 | 269 |
| R: 5'-TTCCGGTGTGCCATCTCCATCA-3' | 1004-1025 | |||
| Cytochrome c | XR_034017 | F: 5'-GGAGGCAAGCATAAGACTGG-3' | 219-239 | 214 |
| R: 5'-GTCTGCCCTTTCTCCCTTCT-3' | 413-432 | |||
| Bmp15 | NM_009757 | F: 5'-CTGACGACCCTACATTGCCCT-3' | 467-487 | 232 |
| R: 5'-TGTACATGCCAGGAACCTCTGG-3' | 677-698 | |||
| Gdf9 | NM_008110 | F: 5'-TCCCAAACCCAGCAGAAGTC-3' | 426-445 | 195 |
| R:5'-GGAGGAGGAAGAGGCAGAGTTG-3' | 609-620 | |||
| GAPDH | NM_008084 | F: 5'-AGGTTGTCTCCTGCGACTTCA-3' | 843-903 | 216 |
| R: 5'-GGGTGGTCCAGGGTTTCTTACT-3' | 1048-1068 | |||
| beta-actin | NM_007393 | F:5'-GAGACCTTCAACACCCCAGC-3' | 452-471 | 263 |
| R:5'-ATGTCACGCACGATTTCCC-3' | 696-714 |
F, forward; R, reverse