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. 2010 May 28;10:155. doi: 10.1186/1471-2180-10-155

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Copyright ©2010 Marvig and Blokesch; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PCR fragments can serve as donor DNA. V. cholerae wild-type strain A1552 was induced for natural competence on crab shell fragments and scored for its transformation frequency (Y-axis). Provided donor DNA was derived either from strain A1552-LacZ-Kan as a positive control (2 μg gDNA; lane 1), or from a PCR reaction according to IV in Fig. 3A. PCR-derived DNA was purified before administered to the bacteria (lane 3; 200 ng). The negative control, with no donor DNA provided, is shown in lane 2. Average of at least three independent experiments.