Table 1.
Oligonucleotides used in this study
| Primer name | Sequence |
|---|---|
| NheI-lacZ-start | 5'-PCGCGCTAGCAAAGGCGTTATTGGCTTGTTGC-3' |
| LacZ-end-SalI | 5'-PCGCGTCGACGCTTTCACACGTAAGGTGAGC-3' |
| Tfm-II-1000 | 5'-CGGGAAGCTAGAGTAAGTAGTTCG-3' |
| Tfm-II+1000 | 5'-CGTTCCATGTGCTCGCCGAGGCG-3' |
| Tfm-II-gDNA-1000 | 5'-AAGCTTCCTGCTTGGAAGAAATGGC-3 |
| Tfm-II-gDNA+1000 | 5'-CGGTGTATCTGTGGCAACGGTTTC-3' |
| Tfm-II-2000 | 5'-CCCCCCTGACGAGCATCACAAAAATCG-3' |
| Tfm-II+2000 | 5'-CTGACGCGCCCTGACGGGCTTGTCTGC-3' |
| Tfm-II-gDNA-2000 | 5'-GAAACCGACGAAGGTGTGTTGATC-3' |
| Tfm-II-gDNA+2000 | 5'-CGCAACCGGATTGGTGCGCTATTTTGGC-3' |
| KanR-500flank-up | 5'-GCGCTTTATCAACACGCTGAATTGC-3' |
| KanR-500flank-down | 5'-ACGCGAAGATCGTCACATTCCACAC-3' |
| KanR-250flank-up | 5'-TGCTTGATGAAGATGGCGCGCCG-3' |
| KanR-250flank-down | 5'-CATCTTGCTGCCATTGAGGCAGCG-3' |
| KanR-100flank-up | 5'-ATGTGATGGATGAAGCAAGCATGCG-3' |
| KanR-100flank-down | 5'-ATTCATGCTCTGGCAACATTGGCAGC-3' |