Figure 6.

Vpu and ROD10 Env have no effect on TfR distribution. HeLa cells were either mock treated or transfected with 2 μg Vphu-HcRed or 2 μg ROD10 Env expression plasmids, permeabilized and stained with specific antibodies against human transferrin receptor (TfR) or HIV-2 Env, or visualized by HcRed fluorescence, and analyzed by confocal microscopy. TfR was found at the cell surface and in a perinuclear concentration, and its distribution was unaltered by expression of either viral protein. Scale bars represent 10 μm.