Table 1.
Characteristics of 25 newly developed sugar beet microsatellite markers.
| Micro-satellite marker1 | EMBL Accession number | Forward primer (5'-3') Reverse primer2 (5'-3') |
Repeat motif3 | Annealing Temp | Predicted size | Quality4 |
|---|---|---|---|---|---|---|
| Bvv01 | [EMBL:AM410749] | CCATATGGAGGGGTAGAGCA | (GGA)4-1(GGT)7 | 55 | 105 | 1 |
| GTTTGCACCATAGGCACCACCACTTG | ||||||
| Bvv10 | [EMBL:AM410750] | CTTTGAGAATTGAGATACTATG | (CA)56-3 | 52 | 212 | 1 |
| GTTTGTCTGGACGCAAGCACAC | ||||||
| Bvv155 | [EMBL:AM410751] | TGCTGACCTTGCAGTTAATAAGTT | (CAA)34-7 | 54 | 298 | 1 |
| GTTTCATGTGATGGCTTGCTTTCTAA | ||||||
| Bvv17 | [EMBL:AM410752] | CGACGCCTTTTTGAAGGAATAGGAT | (GAT)12-3 | 57 | 128 | 1 |
| GTTTCACCCCTGGGTCCTGATCTACAAC | ||||||
| Bvv186 | [EMBL:AM410753] | CACCATAACCGCCCCCACCATAAT | (GCC)3(ACC)3 | 60 | 208 | 1 |
| GTTTCTTGGCCGTAGGGTAAGGGTCAACTA | ||||||
| Bvv21 | [EMBL:AM410754] | TTGGAGTCGAAGTAGTAGTGTTAT | (GGC)13-5 | 53 | 250 | 1 |
| GTTTATTCAGGGGTGGTGTTTG | ||||||
| Bvv22 | [EMBL:AM410755] | CTATGCATCGCCCAATAATTACTTAA | (CCG)5(CCA)5 | 52 | 210 | 1 |
| GTTTATATAACACTGCTTATTTAATGTCC | ||||||
| Bvv23 | [EMBL:AM410756] | TCAACCCAGGACTATCACG | (GA)16 | 50 | 109 | 1 |
| GTTTACTGACAAAGCAAATGACCTACTA | ||||||
| Bvv257 | [EMBL:AM410757] | GAAACCACATAAAAACCCCTCTTA | (TCA)10 | 51 | 121 | 2 |
| GTTTCAAGTAGTCCCGTTAACATCTGA | ||||||
| Bvv27 | [EMBL:AM410758] | GGGTTCATCATCATCCTTATCATT | (TCA)13-3, (TCA)35-10 | 54 | 310 | 1 |
| GTTTACGCTCCTCCATCATCAGACCA | ||||||
| Bvv30 | [EMBL:AM410759] | TGTGCCCAAAATCCTGAA | (GA)31 | 51 | 183 | 1 |
| GTTTAATTGGCTGGGTAAAAGAGA | ||||||
| Bvv31 | [EMBL:AM410760] | AGAAGCCTTTAAAATCCAACT | (CA)14, (GTAT)69 | 49 | 461 | 2 |
| GTTTACAGCGTCTCACCATAAGT | ||||||
| Bvv32 | [EMBL:AM410760] | AGAAGCCTTTAAAATCCAACT | (CA)14 | 50 | 142 | 2 |
| GTTTACATATGGAACTTTAATGAACAAGTGATAT | ||||||
| Bvv37 | [EMBL:AM410761] | TGGACGCCATATTAGAAGAT | (GT)27 | 50 | 216 | 1 |
| GTTTATACAAATGAATATGAGAATACTG | ||||||
| Bvv43 | [EMBL:AM410762] | TGACACTCTTCTTTGCAACACATAA | (GT)96-18 | 54 | 257 | 2 |
| GTTTGTAAATGTTGCAAAATATTGGTAT | ||||||
| Bvv45 | [EMBL:AM410763] | GTATAGCAAAAGTCATTTTGTTTGTGT | (GT)14-1, (CGCA)8 | 55 | 230 | 1 |
| GTTTCTCGGCCTTCCCTTTCTAATGTCTAG | ||||||
| Bvv48 | [EMBL:AM410764] | GGCTTCCCTAGACAACC | (GT)24 | 50 | 209 | 1 |
| GTTTATAGGCAAATGAATGAGG | ||||||
| Bvv51 | [EMBL:AM410765] | AGCAAAACTTATCTCAAATCTGG | (TG)9(AG)32-1 | 51 | 272 | 1 |
| GTTTGTCTACCGTGGCTGTGC | ||||||
| Bvv53 | [EMBL:AM410766] | CATGTCGAGGAGTGAGTTCAGGAA | (GT)17, (GA)35 | 53 | 226 | 1 |
| GTTTCAACTATAGGTGCATCTTTTAC | ||||||
| Bvv54 | [EMBL:AM410767] | ATCTGCATGCCGTCACTC | (TC)12(AC)12 | 52 | 279 | 1 |
| GTTTCACTGTACCTTCGAATGTTAG | ||||||
| Bvv57 | [EMBL:AM410768] | CATTACCATGGGAACGAA | (GT)25 | 50 | 232 | 1 |
| GTTTAAGGGATACAATGTTAGTTATGAA | ||||||
| Bvv60 | [EMBL:AM410769] | AAGAATGCTTCAACTTTTTCATGG | (CAT)7, (CAT)7, (CAT)11-1 | 52 | 256 | 1 |
| GTTTAGGGTCGGATATAAGAGGGAGTGG | ||||||
| Bvv61 | [EMBL:AM410770] | ATGGGAGAATATTGGTGACA | (GAA)22 | 49 | 200 | 1 |
| GTTTGCCACAAATCATCTCTACTAA | ||||||
| Bvv62 | [EMBL:AM410771] | ATGGCAATGCGCAGAATAACC | (CAG)11-2 | 54 | 155 | 1 |
| GTTTGCTGAGGAGGCTGCATTTGTT | ||||||
| Bvv64 | [EMBL:AM410772] | TTTTTGGGAGTTTCATCACTACTTT | (CT)18-1(CA)19 | 51 | 207 | 2 |
| GTTTCATATAAGGGGAGTCTTCTCACAA |
1 In bold the 12 markers that have been used to genotype 40 cultivars (1200 plants) (see Table 2). They were amplified separately but combined before analysis on an ABI sequencer, as follows: multiplex 1 consisted of markers Bvv15, Bvv30, and Bvv64; multiplex 2 of Bvv17, Bvv43, and Bvv61; multiplex 3 of Bvv 51, Bvv 53, and Bvv60; multiplex 4 of Bvv 21, Bvv23, and Bvv32
2 GTTT is a pigtail [24]
3 the number after the minus sign is the number of imperfect repeats. For instance, (CA)56-3 means that the microsatellite repeat covers of length of 56 (CA) repeat units, but of these 3 are not (CA).
4 according to Smulders et al. [25]
5 The sequence of the forward primer of Bvv15 was found in cDNA clone EO12340
6 The sequence of the cloned Bvv18 fragment was found in cDNA clone EG551697
7 The sequence of the cloned Bvv25 fragment was found in BAC clone ED032383