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. 2010 Jun 1;107(24):11062–11067. doi: 10.1073/pnas.1006301107

Fig. 3.

Fig. 3.

CXCR4 antagonism prevents maturation of OPCs and remyelination. (A) Confocal IHC analysis of CC from mice after 6 wk of CPZ ingestion then 2 wk of treatment with PBS (Left) or AMD3100 (Right, AMD). Sections were stained for CXCR4 (green), NG2 (red), and nuclei (blue). Note linear organization of NG2-negative, oligodendrocyte nuclei in PBS-treated specimen (arrowheads). (Inset) Isotype control. (Scale bar, 25 μm.) (B) Quantitation of CXCR4+NG2+ cells within rostral, central and caudal sections of the CC in naive mice (white bars) and after 6 (gray bars) and 12 (black bars) wk of CPZ exposure; n = 6 images taken from four mice/group. *, P < 0.05. (C) Sections from CC of PBS- (Left) or AMD3100- (Right, AMD) treated mice were stained for CNPase (green) and nuclei (blue). (Inset) Isotype control. (Scale bar, 25 μm.) (D) Quantitation of CNPase+ cells within rostral, central, and caudal sections of the CC in naive mice (white bars) and after 6 (gray bars) and 12 (black bars) wk of CPZ exposure; n = 6 images taken from four mice/group. *, P < 0.05. (E) LFB myelin staining of CC in mice at 0 and 6 wk of CPZ treatment and after 6 wk of CPZ treatment plus 2 wk of treatment with PBS or AMD3100 (AMD). (F) IHC analysis of MBP expression (red) within CC of naive mice (Upper Left) and in mice treated with 4 wk of PBS (Lower Left) or AMD3100 (Lower Right, AMD) after 6 wk of CPZ ingestion. Nuclei counterstained with DAPI (blue). (Scale bar, 50 μm.) (G) Quantitation of MBP expression within all regions of the CC in mice treated with 4 wk of PBS or AMD3100 (AMD) after 6 wk of CPZ ingestion; n = 6 images taken from four mice/group. *, P < 0.05.