Fig. 1.
Suppression of aromatase activity/expression in H295R cells by LBH589. (A) H295R cells were treated with LBH589 for 24 h. After treatment, the cells were washed with PBS, and aromatase activity was measured by the [3H] H2O release assay. Student's t test was used for statistical analysis compared with the vehicle control. **P < 0.001. (B) MCF7 and MCF7/Her2 cells were treated with LBH589 (50 nM) or with DMSO as a control for 24 h. After treatment, total RNA was isolated; 5 μg of total RNA was used for real-time qPCR for quantifying aromatase gene expression. β-actin mRNA was amplified as an internal control. All samples were run in triplicate, and SDs were calculated. (C) After treatment with LBH589 (50 nM) for 24 h, H295R cells were lysed and applied for Western blot analysis using aromatase antiserum.