Fig. 1.

Vacuolation and vascular proliferation in NesKO and KO mice. (A) No pathology is observed in H&E staining of brain sections of CT animals (n = 5). (B and C) In contrast, both late-stage NesKO (B, n = 5) and KO (C, n = 5) mice show marked vacuolation in the VN (black arrows; Inset shows higher magnification of indicated area) and, to a lesser extent, in deep cerebellar nuclei (white arrow). (D–F) Vascular proliferation, as assessed by laminin staining, was increased around the VN in NesKO (E) and KO (F) mice compared with CT (D). (Scale bar, A–F, 125 μm; Inset, 25 μm.)