Fig. 3.

Neuronal loss in affected brain areas of KO mice. (A–C) NeuN labeling (green) and Topro-3 counterstain (red) in the cerebellum of CT (A) and late-stage KO (B) mice. Colocalization of both labels is shown in yellow. (C) Quantification of NeuN-positive cells; the number of cells was reduced in lesion foci (KO lesion, representative area denoted by white rectangle), as well as in adjacent regions of the cerebellum (KO, black rectangle) of late-stage KO mice (B) compared with CT mice (A). *P < 0.05,*** P < 0.001 vs. CT. Topro-3–positive/NeuN-negative cells (probably microglia) were increased in lesioned areas of KO mice (B) (D–F) Significant decrease in NeuN-positive cells in the VN of KO mice (E) compared with CT mice (D and F for quantification), *P < 0.05 vs. CT. (G–I) Loss of NeuN-positive cells in the OB of late-stage KO mice (H) compared with CT mice (G, quantification in I). An increase in Topro-3–positive/NeuN-negative cells was observed in the OB of KO mice (H). **P < 0.01 vs. CT. (Scale bar, A–H, 100 μm.)