Fig. 5.

Light microscopy and EM changes in cerebellar cortex and OB of KO Mice. (A and B) Light microscopy demonstrates granular (gc), Purkinje (PC), and molecular layers (m) in (A) anterior and (B) posterior vermis. Spongiform degeneration (arrowheads) is evident only in the posterior lobules, in addition to vascular spaces, which exist in both areas. (C) Abnormal mitochondria with compact cristae (arrows; higher magnification in D) are present in presynaptic basket cell nerve termini adjacent to the cell body of a Purkinje cell (PC). (E) Intracellular edema with swelling and lysis of cytoplasmic organelles is evident in a degenerating neuronal cell body (N, nucleus) from the periglomerular external plexiform layer of the OB. (F, Inset) Swollen mitochondria (m) and a portion of a nucleus (N) at higher magnification. (Scale bars, A, 35 μm; B, 35 μm; C, 1 μm; D, 0.5 μm; E, 1 μm; F, 1 μm.)