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eEF3/ATP releases mRNA from the PoTC. The purified polysomes (Fig. S1C) (15 pmol) were incubated for 3 min at 30 °C in buffer 3/150 with additions, as shown below each sedimentation profile. MgCl2 was added to 25 mM to stop the reaction. The percentage of polysome bound mRNA that was released is indicated above the polysome region. The complete reaction mixture (E) contained 50 μM ATP, 0.5 μM eEF3, and 1 mM PUR in buffer 3/150.
