Fig. 2.
HGF receptor Met expression is elevated in human ESCC tumors and activated upon EGFR and p53R175H overexpression. (A) Met IHC staining results of 72 ESCC human tumor microarray samples. (B) Representative images of two ESCC human tumor samples with matched normal mucosa stained for Met. (Scale bar: 100 μm.) (C and D) Levels and phosphorylation status of the indicated proteins measured by Western blotting in whole-cell lysates of EPC-hTERT, EPC-hTERT-EGFR-puro, EPC-hTERT-p53R175H-neo, and EPC-hTERT-EGFR-p53R175H cells (C) or a panel of ESCC cell lines (D).