Fig. 5.

Expression of constitutively active Met enhances invasion of transformed esophageal epithelial cells. (A) Phase contrast images of EPC-hTERT-p53^R175H-TPR-Met or EPC-hTERT-p53^R175H-puro grown in 2D on tissue culture plastic. Images a and b represent independently generated cell lines with the same genotype. (Scale bar: 100 μm.) (B) Western blot of EPC-hTERT-p53^R175H-TPR-Met or EPC-hTERT-p53^R175H-puro whole-cell lysates for detection of wild-type Met (145 kDa), TPR-Met (65 kDa), and phosphorylation status. (C and D) H&E-stained sections of organotypic culture of EPC-hTERT-p53^R175H-TPR-Met or EPC-hTERT-p53^R175H-puro cells seeded above matrices containing FEF3 (C and D) or FEF4736 (D) fetal esophageal fibroblasts. (Scale bar: 100 μm.)