Fig. 2.

Maternal potentiation was exhibited in call duration and latency. (A) Representative spectrogram of a multicomponent call and its segmentation. (Left) Call with segment lines, calculated from amplitude envelope shown in overlay. (Center) Extracted instantaneous frequencies. (Right) Histogram of frequency counts. (B) Mean duration of each call rose on average from isolation 1 to isolation 2 (P < 0.001), and call durations were longer on average across isolation periods from pups born to heterozygous dams (P = 0.012). (C) Call latency strongly decreased following reunion on average across all genotypes (P < 0.001). Only the WT pup, heterozygous dam group did not statistically significantly increase in call duration or decrease in latency.