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. 2010 Jun 1;107(24):10978–10983. doi: 10.1073/pnas.1006545107

Fig. 2.

Fig. 2.

Four independent insulin B-chain responsive T cells recognize the peptide bound to IAg7 in register 3. Triplicate culture wells of the BDC 12-4.1, AS91, AS150, and I.29 insulin B-chain responsive CD4+ T-cell hybridomas were stimulated with an immobilized anti-Cβ Mab (five experiments), NOD spleen cells with or without 50 μg/mL of soluble insulin B:9–23 peptide (two experiments), ICAM/B7+-infected insect cells expressing IAg7 linked to the control hen egg lysozyme (HEL) peptide or to the unmutated B:12–23 peptide (three experiments), or ICAM/B7+-infected insect cells expressing IAg7 linked to each of three trapped versions (Fig. S6) of the insulin B:10–23 peptide (three experiments). The responses of the hybridomas were assessed by IL-2 production, and the average response and SEM is shown.