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Expression of chromosomal antR-lacZ transcriptional (A) and translational fusion (B) in the LasR-RhlR-QscR triple-mutant strain DA15. The results of two independent experiments are shown. Open symbols indicate activity in the absence of added acyl-HSL, and closed symbols indicate activity in the presence of 2 μM C10-HSL. β-galactosidase activity is given as relative light units normalized to OD600.
