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A representative experiment showing AntR protein levels in the LasR-RhlR-QscR triple-mutant strain DA15 expressing AntR with an amino terminal VSV-G tag. Cells were grown with (closed squares) or without (open squares) 2 μM C10-HSL. Levels were monitored by Western blotting with VSV-G antiserum, and the intensity of the bands was quantitated as described in Materials and Methods. AntR levels are the band intensities in the Western blot.
