Table 2.
Bacterial strains used
| Strain or plasmid | Relevant properties | Source or reference |
| E.coli | ||
| DH5α | F−, φ80dlacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk−, mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | Invitrogen |
| S17-1 | recA pro hsdR RP4-2-Tc::Mu-km::Tn7 | (46) |
| P. aeruginosa | ||
| PAO1 | WT | (42) |
| DA15 | PAO1 derivative, ΔlasR, ΔrhlR, ΔqscR | (47) |
| SC20 | DA15 ΔPA1136 | This study |
| SC21 | SC24 ΔpqsR | This study |
| SC22 | SC24 ΔtrpE | This study |
| SC23 | SC24 ΔkynA | This study |
| SC24 | DA15 with a catB-lacZ transcriptional fusion at the attB site | This study |
| SC26 | DA15 with a antR-lacZ transcriptional fusion at the attB site | This study |
| SC27 | DA15 with a antR-lacZ translational fusion at the attB site | This study |
| SC28 | PAO1 with a antR-lacZ transcriptional fusion at the attB site | This study |
| SC29 | PAO1 with a antR-lacZ translational fusion at the attB site | This study |
| SC30 | DA15 with a VSV-G epitope sequence tagged to the N terminus of AntR | This study |
| SC31 | SC24 with a VSV-G epitope sequence tagged to the N terminus of AntR | This study |
| Plasmids | ||
| pEXG2 | Gene-replacement vector; sacB Gmr | (43) |
| mini-CTX-lacZ | Integrative reporter vector; Tcr | (44) |
| mini-CTX-lacZ-EB | Integrative reporter vector; Tcr | Brutinel and Yahr, University of Iowa |
| pFLP2 | Source of Flp recombinase; Apr/Cbr | (45) |
km, kanamycin resistance; Apr, ampicillin resistance; Cbr, carbenicillin resistance; Tcr, tetracycline resistance; Gmr, gentamicin resistance.