Skip to main content
. 2010 May 20;107(23):10360–10365. doi: 10.1073/pnas.0912081107

Fig. 1.

Fig. 1.

In vivo imaging of glycans in early zebrafish embryos using microinjection of azidosugars and detection with copper-free click chemistry. (A) Metabolic labeling of mucin-type O-glycans with azidosugars via the GalNAc salvage pathway. The enzymatic transformations shown are catalyzed by (i) nonspecific esterases, (ii) GalNAc-1-phosphate kinase, (iii) UDP-GalNAc pyrophosphorylase, and (iv) ppGalNAcTs and other glycosyltransferases. (B) Two-step strategy for imaging glycans in vivo. (C and D) Zebrafish embryos were microinjected with UDP-GalNAz (C, top), GalNAz (D, top), UDP-GalNAc (C, bottom), or no sugar (D, bottom), along with the tracer dye rhodamine-dextran, allowed to develop to 7 hpf, reacted with DIFO-488 (100 μM, 1 h), and imaged by confocal microscopy. Shown are maximum intensity z-projection images. Green, DIFO-488; red, rhodamine-dextran. (Scale bar: 200 μm.)