Table 1.
MSDRs for 5 days in DD
| Genotype | sLNv | lLNv | LNd | DN1 | Total flies, n | Rhythmic flies (%) | Period, h | Power | Trough phase | Phase concentration |
| Oregon-R | 15 | 11 (73) | 23.7 ± 0.1 | 124 ± 15 | −10.8 | 0.96 | ||||
| UAS-CYCΔ/+ | 17 | 12 (71) | 23.6 ± 0.2 | 88 ± 12 | 11.9 | 0.91 | ||||
| Mz520-GAL4/+ | 32 | 21 (66) | 23.4 ± 0.1 | 102 ± 12 | −11.4 | 0.94 | ||||
| c319-GAL4/+ | 32 | 25 (78) | 23.9 ± 0.1 | 126 ± 10 | −10.9 | 0.96 | ||||
| Clk4.4F-GAL4/+ | 32 | 25(78) | 23.2 ± 0.3 | 111 ± 11 | −10.5 | 0.88 | ||||
| cry-GAL4_2/+ | 17 | 13 (76) | 23.9 ± 0.2 | 91 ± 17 | −10.4 | 0.94 | ||||
| fruGAL4/+ | 26 | 17 (65) | 23.9 ± 0.4 | 80 ± 9 | −11.0 | 0.68 | ||||
| Pdf-GAL80/+;fruGAL4/+ | 18 | 11 (61) | 24.1 ± 0.4 | 109 ± 17 | −11.1 | 0.82 | ||||
| cry-GAL80 fruGAL4/+ | 14 | 10 (71) | 23.3 ± 0.2 | 124 ± 17 | −10.2 | 0.94 | ||||
| Pdf-GAL80/+ | 16 | 14 (88) | 23.7 ± 0.1 | 121 ± 14 | −11.1 | 0.99 | ||||
| Pdf01 | 27 | 4 (15) | 25.2 ± 1.4 | 51 ± 18 | 10.0 | 0.54† | ||||
| fruGAL4 | 30 | 2 (7) | 28.5 ± 2.8* | 43 ± 5 | 4.9 | 1.00† | ||||
| Mz520-GAL4/UAS-CYCΔ | + | + | 12 | 0 (0) | — | — | — | — | ||
| Mz520-GAL4 Pdf-GAL80/UAS-CYCΔ | 14 | 14 (100) | 23.2 ± 0.1* | 84 ± 13 | −10.9 | 0.95 | ||||
| c319-GAL4/UAS-CYCΔ | + | 27 | 6 (22) | 23.3 ± 0.9 | 51 ± 8 | −7.1 | 0.32† | |||
| UAS-CYCΔ/+;Clk4.4F-GAL4/+ | + | 27 | 4 (15) | 24.7 ± 1.9 | 62 ± 26 | −5.5 | 0.79† | |||
| UAS-CYCΔ/+;cry-GAL4_2/+ | + | + | + | + | 16 | 0 (0) | — | — | — | — |
| UAS-CYCΔ/+;fruGAL4/+ | + | + | + | 37 | 9 (24) | 23.0 ± 1.0 | 62 ± 13 | −9.0 | 0.62† | |
| Pdf-GAL80/UAS-CYCΔ;fruGAL4/+ | + | + | 27 | 8 (30) | 22.8 ± 0.7 | 51 ± 7 | −7.9 | 0.71† | ||
| UAS-CYCΔ/+;cry-GAL80 fruGAL4/+ | 26 | 13 (50) | 23.4 ± 0.3 | 117 ± 15 | −10.3 | 0.92 | ||||
| C155-elav-GAL4 per0 w;UAS-per2/+ | + | + | + | + | 24 | 19 (79) | 24.4 ± 0.3* | 93 ± 8 | −10.9 | 0.85 |
| C155-elav-GAL4 per0 w;Pdf-GAL80/+;UAS-per2/+ | + | + | 26 | 1 (4) | 21.7 | 38 | −2.6 | 1.00† | ||
| per0 w;UAS-per16/+ | 18 | 1 (6) | 30.0 | 25 | 0.5 | 1.00† | ||||
| per0 w;Mz520-GAL4/+;UAS-per16/+ | + | + | 43 | 26 (60) | 24.0 ± 0.5 | 78 ± 6 | 10.3 | 0.41† | ||
| per0 w;Mz520-GAL4 Pdf-GAL80/+;UAS-per16/+ | 7 | 1 (14) | 23.7 | 88 | 11.8 | 1.00† | ||||
| per0 w;c319-GAL4/+;UAS-per16/+ | + | 30 | 10 (33) | 23.8 ± 0.5 | 75 ± 12 | −8.9 | 0.82 | |||
| per0 w;Clk4.4F-GAL4/UAS-per16 | + | 22 | 4 (18) | 24.5 ± 0.3* | 57 ± 12 | −9.6 | 0.90† | |||
| per0 w;Mz520-GAL4 c319-GAL4/+;UAS-per16/+ | + | + | + | 36 | 20 (56) | 23.0 ± 0.3* | 87 ± 9 | −11.2 | 0.74 | |
| per0 w;UAS-per16/cry-GAL4_2 | + | + | + | + | 32 | 19 (59) | 22.5 ± 0.6* | 88 ± 11 | −10.2 | 0.69 |
| per0 w;UAS-per16/fruGAL4 | + | + | + | 33 | 20 (61) | 25.8 ± 0.4* | 82 ± 10 | −11.0 | 0.77 |
Genotypes in bold show significant (χ2 test, P < 0.05) decreases in the fraction of rhythmic flies compared with Oregon-R. Clock neurons expressing UAS-CYCΔ or UAS-per in each genotype are indicated by a +. All per0-rescue flies except C155-elav-GAL4 per0 w;Pdf-GAL80/+;UAS-per2/+, per0 w;Mz520-GAL4 Pdf-GAL80/+;UAS-per16/+, and per0 w;Clk4.4F-GAL4/UAS-per16 show significant (χ2 test, P < 0.05) increases in the fraction of rhythmic flies compared with per0 w;UAS-per16/+.
*Significant (Kruskal–Wallis ANOVA test, P < 0.05) period difference compared with Oregon-R.
†Low phase concentration (Rayleigh test, P > 0.01).