Fig. 5.

NOX2 activity diminishes the reductive capacity of the phagosome. Phagosomal reductive capacity was assessed by measurement of fluorescence liberated through reduction of a modified fluorogenic cystine-based reagent covalently bound to IgG-coupled experimental particles in resting (A and B) and IFNγ-activated (C and D) BMMØs. (A and C) Real-time representative traces. (B and D) Averaged rates relative to DMSO-treated WT samples from three independent experiments. Error bars denote SEM. P values were determined by ANOVA.