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. 2010 May 24;107(23):10538–10542. doi: 10.1073/pnas.1000525107

Fig. 1.

Fig. 1.

Generation of Mkx mutant mice. (A) Diagram of the Mkx targeting construct. Blue and red arrows (a–e) show genomic PCR primers for genotyping. White box, UTR; Black box, coding region; DT-A, diphtheria toxin A; WT, wild-type allele; TA, targeted allele. (B) Genomic PCR of wild-type and Mkx mutant mice for genotyping using primers a, b and c. (C) Whole-mount in situ hybridization of Mkx (Upper) and whole-mount visualization of Venus signals (Lower) in E13.5 forelimb and tail of wild-type or Mkx mutant embryos. (D) Immunohistochemistry for anti-myosin heavy chain (MF20; red) and visualization of Venus in cryosection of E16.5 tail of a Venus knockin Mkx heterozygous embryo. (Scale bar, 100 μm.) (E) RT-PCR analysis for Mkx and Gapdh of Achilles tendon in wild-type and Mkx mutant mice.