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. Author manuscript; available in PMC: 2010 Jun 24.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2006 Mar 24;291(2):G229–G237. doi: 10.1152/ajpgi.00092.2006

Fig. 2.

Fig. 2

Hepcidin is up-regulated in the liver of Hfe-deficient mice in response to iron-loading. Hepcidin gene expression in the liver of mice fed a standard diet (SD; light gray) or an iron-enriched diet (+Fe; dark gray) from the C57BL/6 (A) and 129/SvEvTac background (B). Hepcidin mRNA expression was quantified by qRT-PCR and normalized to β-actin. The hepcidin/β-actin ratio is shown. The results are presented as means ± SD (n = 5–7 mice/group). Statistical analysis was performed by 1-way ANOVA; *P < 0.01, and **P < 0.001 for comparison with relevant saline-treated mice (SD diet).