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. Author manuscript; available in PMC: 2010 Jun 24.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2006 Mar 24;291(2):G229–G237. doi: 10.1152/ajpgi.00092.2006

Fig. 5.

Fig. 5

Hepatic Fp1 regulation by iron is independent of hepcidin levels. Fp1 mRNA levels in the liver (A) and duodenum (B) in control (CTL; light gray), iron-loaded (+Fe; dark gray), and LPS-treated mice (black). The results are presented as means ± SD (n = 6 mice/group). Statistical analysis was performed by 1-way ANOVA; *P < 0.01, and **P < 0.001 for comparison with relevant control mice. Fp1 mRNA expression was quantified by qRT-PCR and normalized to β-actin. The Fp1/β-actin ratio is shown. C: effect of iron, LPS, IL-6, and TNF-α on Fp1 expression by primary murine hepatocytes. Each symbol represents 1 experiment in which 3 samples of primary murine hepatocytes were incubated with culture medium, FAC, LPS, or the cytokines indicated. The results are expressed as fold changes of Fp1 mRNA normalized to β-actin of treatments compared with CTL.