Figure 3. Impact of an Acrolein Adduct on Thermodynamic Stability of the DNA Host Duplex.
Comparison of excess heat capacity profiles determined for the α-OH-PdG·C (red) and α-OH-PdG·A (magenta) duplexes relative to the corresponding G·C parent (blue) and G·A mismatch (navy) 11-mers. The inset furnishes van’t Hoff analyses of the optically-derived concentration-dependent dissociation profiles for these four duplexes. The “undamaged” G·A mismatch and adduct-containing 11-mers paired with dA or dC significantly disrupt cooperative duplex dissociation with differential destabilization ranked as follows: G·A < α-OH-PdG·A < α-OH-PdG·C.