Table III.
A. Thermodynamic Dissociation Parameters of Canonical, Mismatch, and Adduct-Containing Duplexes Extrapolated to 25.0 °C Following ΔCp Corrections. B. Impact of α-OH-PdG and Opposite Base on the ΔCp-Corrected Differential Thermodynamic Destabilization.
| A | Duplex Acronym |
ΔG (kcal·mol−1) |
ΔHcal (kcal·mol−1) |
TΔS (kcal·mol−1) |
|---|---|---|---|---|
| Canonical G·C | 12.6 | 53.2 | 40.6 | |
| Mismatch G·A | 9.9 | 53.8 | 43.9 | |
| α-OH-PdG·C | 7.1 | 38.1 | 31.0 | |
| α-OH-PdG·A | 8.2 | 45.5 | 37.4 | |
| B | Duplex Acronym |
ΔΔG (kcal·mol−1) |
ΔΔH (kcal·mol−1) |
ΔTΔS (kcal·mol−1) |
| G·C to α-OH-PdG·C | − 5.5 | − 15.1 | − 9.6 | |
| G·C to α-OH-PdG·A | − 4.4 | − 7.6 | − 3.3 | |
| G·C to G·A | − 2.7 | + 0.6 | + 3.3 | |
| G·A to α-OH-PdG·A | − 1.7 | − 8.3 | − 6.6 | |
| α-OH-pdG·C to α-OH-PdG·A |
+ 1.1 | + 7.4 | + 6.3 | |
A. The thermodynamic parameters are reported at 25.0 °C following ΔCp correction as described in Materials and Methods. B. The values of ΔTm, ΔΔG, ΔΔH, and ΔTΔS are calculated by subtracting the respective reference duplex dissociation parameter from that of the damaged and/or mismatched duplex.