. Author manuscript; available in PMC: 2011 Oct 1.

Published in final edited form as: Dig Liver Dis. 2010 Mar 20;42(10):709–717. doi: 10.1016/j.dld.2010.02.008

Table 2.

Immunohistochemical evaluation of the percentage of cholangiocytes positive for VEGF-A, and VEGFR-2 in liver sections.

Parameters	BDL + BA control feeding 1 week	BDL + TC feeding 1 week	BDL + HAL + BA control feeding + NaCl daily injections 1 week	BDL + HAL + TC feeding + NaCl daily injections 1 week	BDL + HAL + TC feeding + wortmannin daily injections 1 week
VEGF-A	70.4 ± 0.2	73.7 ± 3.0	38.0 ± 1.4^*	80.2 ± 2.1	50.3 ± 2.0^*
VEGFR-2	45.2 ± 3.0	48.5 ± 2.5	34.3 ± 1.8^*	46.8 ± 1.8	28.3 ± 1.6^*

HAL decreased the percentage of cholangiocytes positive for VEGF-A, and VEGFR-2. Taurocholic acid feeding to BDL + HAL rats prevented HAL-induced decrease in VEGF-A and VEGFR-2 protein expression, decrease that was blocked by the simultaneous administration of wortmannin.

p<0.05 vs. the corresponding value of BDL rats fed bile acid control diet.

Data are mean ± SEM of cumulative values obtained from the evaluation of 10 different fields from three different sections (6 sections analyzed per group) for each group. BA = bile acid; BDL = bile duct ligation; HAL = hepatic artery ligation; TC = taurocholic acid.