Table 4.
Measurement of basal and secretin-stimulated bile flow, bicarbonate concentration and secretion in 1-week BDI rats and rats that [immediately after BDI + hepatic artery ligation (HAL)] were treated by daily IP injections of NaCl or wortmannin in the absence or presence of bile acid control or 1% taurocholic acid (TC) feeding for 1 week.
| Bile Flow | Bicarbonate Concentration | Bicarbonate Secretion | ||||
|---|---|---|---|---|---|---|
| Treatment | Basal (μl / min / Kg BW) | Secretin (μl / min / Kg BW) | Basal (mEq / Liter) | Secretin (mEq / Liter) | Basal (μEq / min / Kg BW) | Secretin (μEq / min / Kg BW) |
| BDI + bile acid control diet (n = 8) | 108.5 ± 4.2 | 162.9 ± 10.4 * | 36.4 ± 2.1 | 53.3 ± 5.5 * | 4.0 ± 0.3 | 8.6 ± 1.0 * |
| BDI + 1% TC (n = 8) | 164.3 ± 16.3 # | 240.3 ± 25.7 * | 44.9 ± 2.2 # | 56.9 ± 2.1 * | 7.4 ± 0.9 # | 13.8 ± 1.6 * |
| BDI + HAL + bile acid control diet (n = 6) | 91.2 ± 9.7 | 104.2 ± 9.1 ns | 40.2 ± 2.1 | 40.6 ± 1.6 ns | 3.6 ± 0.4 | 4.2 ± 0.3 ns |
| BDI + HAL + NaCl + 1% TC feeding 1 week (n = 6) | 100.4± 8.5 | 140.9 ± 13.4 * | 35.8 ± 4.9 | 60.6 ± 9.3 * | 3.4 ± 0.6 | 8.2 ± 0.7 * |
| BDI + HAL + wortmannin+ 1% TC feeding 1 week (n = 7) | 88.5 ± 13.4 | 88.5 ± 15.0 ns | 57.6 ± 2.7 | 62.4 ± 3.0 ns | 5.3 ± 0.8 | 5.8 ± 1.1 ns |
When steady spontaneous bile flow was reached (60-70 minutes from the infusion of Krebs Ringer Henseleit (KRH), rats were infused for 30 minutes with secretin followed by a final infusion of KRH for 30 minutes. After the rats were surgically prepared for bile flow experiments, bile was collected every 10 minutes in pre-weighed tubes and used for determining bicarbonate concentration. Data are mean ± SEM.
p < 0.05 vs. corresponding basal value of bile flow, bicarbonate concentration or bicarbonate secretion of BDI rats.
p < 0.05 vs. corresponding basal value of bile flow, bicarbonate concentration or bicarbonate secretion of BDI rats.
vs. corresponding basal value of bile flow, bicarbonate concentration or bicarbonate secretion of BDI rats. Differences between groups were analyzed by the Student unpaired t test when two groups were analyzed and analysis of variance (ANOVA) when more than two groups were analyzed. TC = taurocholic acid.