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. 2010 May 26;30(21):7215–7226. doi: 10.1523/JNEUROSCI.0048-10.2010

Figure 3.

Figure 3.

Effects of manipulations of microtubule acetylation on katanin/spastin-mediated microtubule severing. RFL-6 cells were transfected with katanin or spastin plasmids under conditions of HDAC6 overexpression or tubacin treatment. Top row (a–e) shows microtubules of katanin overexpressors revealed by general tubulin staining, and bottom row (f–j) shows those of spastin overexpressors. High, High-level katanin or spastin overexpressors; Middle, middle-level katanin or spastin overexpressors; Middle + HDAC6, middle-level katanin/spastin overexpressors with HDAC6 coexpression; Middle + Tubacin, middle-level katanin/spastin overexpressors under tubacin treatment; Middle + TSA, middle-level katanin/spastin overexpressors under TSA treatment. In middle-level katanin overexpressors, HDAC6 inhibited severing, whereas tubacin or TSA enhanced severing. Conversely, such effects on severing were not found in spastin overexpressors. Scale bar, 20 μm. k, Quantification of microtubule (MT) levels by general tubulin staining intensity. *p < 0.01 (Student's t test).