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. 2008 Nov 10;19(11):1317–1323. doi: 10.1089/hum.2008.070

FIG. 1.

FIG. 1.

Serum GUSB activity and histochemical stain of liver for GUSB activity. Some normal C57BL/6 mice received neonatal intravenous injection of LTR-intact γ-RV, designated hAAT-cGUSB-WPRE, at 1010 TU/kg 2 or 3 days after birth. Other normal C57BL/6 mice were not treated. (A) Serum GUSB activity. Average serum GUSB activity ± SD was determined for all surviving mice at the indicated age after transduction. The average serum GUSB activity ± 2 SD in nontransduced normal mice (22 to 31 U/ml) is indicated by the shaded region. (B–E) Histochemical assay for liver GUSB activity. Liver biopsies were obtained 4 months after birth and stained for 1 hr for GUSB activity, which results in a red color in the cytoplasm. Nuclei were counterstained with hematoxylin, which results in a blue color. Samples from nontransduced normal C57BL/6 mice (B and C) have a low level of endogenous GUSB activity. Neonatal γ-RV-transduced normal mice (D and E) have several clusters of hepatocytes with high enzyme activity, as indicated by the arrows.