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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2009 Apr 17;47(1):62–71. doi: 10.1016/j.freeradbiomed.2009.04.016

Fig. 2.

Fig. 2

Formation of 5-LO metabolites in A23187-stimulated neutrophils. Neutrophils (1 × 106 cells in 1 ml) suspended in RPMI containing 0.5% FBS were stimulated by addition of A23187 (5 μM) and incubated in 6-well plates for up to 24 h. 5-HETE (○), 5-oxo-ETE (•), and LTB4 (Δ) were measured by RP-HPLC as described under Experimental Procedures.