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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Neurotoxicology. 2009 Dec 5;31(5):562–571. doi: 10.1016/j.neuro.2009.12.002

Figure 5.

Figure 5

Comparative in vitro inhibition of rat hippocampal monoacylglycerol lipase activity by chlorpyrifos oxon and paraoxon. Rat hippocampus was homogenized on ice in 0.32 M sucrose, pH 8.0 with a Polytron at 27,000 rpm. Tissues were centrifuged at 100,000 × g for 60 minutes to obtain a soluble fraction. Aliquots of the soluble fraction were preincubated for 30 minutes with vehicle or one of a range of concentrations of either paraoxon (PO) or chlorpyrifos oxon (CPO) prior to evaluating residual monoacylglycerol lipase (MAGL) activity with 2-oleoyl [3H]glycerol as the substrate (10 μM final concentration).