Expression of PAF complex molecules in Paf1/PD2 knockout and RNAi-treated ESCs. (A): Western blot analysis showed an expression of other PAF complex molecules (Cdc73, Leo1, sKi8, and Ctr9) in wild-type ESCs and Paf1/PD2 heterozygous knockout ESCs. (B): Transient knockdown of Paf1/PD2 protein using specific RNAi at 48 and 72 hours in J1 ESCs. Expression of Paf1/PD2 protein and other PAF complex proteins (Cdc73, Leo1, sKi8, and Ctr9) in Paf1/PD2 RNAi-treated J1 ESCs by Western blot. β-actin served as a loading control. (C): Transient knockdown of Paf1/PD2 protein (NIH3T3 cells) using specific RNAi for both 48 and 72 hours. Expression of Paf1/PD2 protein and other PAF complex proteins (Cdc73, Leo1, sKi8, and Ctr9) in Paf1/PD2 RNAi-treated NIH3T3 cells by Western blot. β-actin served as a loading control. Abbreviations: h, hour; PD2+/−, PD2 heterozygous knockout, Scr, Scrambled RNAi.