Table 1.
Photophysical and photochemical parameters of various azido fluorogens (in ethanol unless otherwise stated)
| λabs,azido (nm)[a] | λabs,amino (nm)[b] | λfl,amino[c] (nm) |
Yield[d] | ΦF[e] | ΦP[f] | ΦB (10−6)[h] | |
|---|---|---|---|---|---|---|---|
| {εmax (M−1 cm−1)} | {εmax (M−1 cm−1)} | {λP}[g] | in PVA | ||||
 |
379 {19,300}[i] |
475 {44,900}[i] |
496 | 24% | 0.003 | ~0.08 {385 nm} |
4.6 |
 |
424 {29,100} |
570 {54,100} |
613 | 65% | 0.025 | 0.0059 {407 nm} |
4.1[k] |
 |
415 {19,100} |
479 | 591 | ~50% | – | ~0.09 {385 nm} |
– |
 |
420 {18,100}[i] |
517 | 611 | – | – | – | 3.4 |
 |
407 {26,700} |
463 {20,000} |
578 | 87% | 0.0062 | 0.017 {385 nm} |
9.2 |
 |
403 {31,300} |
456 {31,100} |
599 | < 30% | 0.18 | 0.085 {385 nm} |
6.2 |
 |
375 {41,000}[j] |
449 | 580 | ~25% | – | ~0.2 {365 nm} |
– |
 |
384 {14,400}[i] |
456 | 539 | ~50% | 0.20 | ~0.2 {365 nm} |
100[l] |
[a]
Peak absorbance and molar absorption for azido fluorogen, and
[b]
amino fluorophore.
[c]
Fluorescence peak of amino fluorophore.
[d]
Overall chemical reaction yield to the fluorescent amino product (see Methods).
[e]
Fluorescence quantum yield of amino fluorophore. DCDHFs become much brighter in rigid environments.20
[f]
Photoconversion quantum yield of azido fluorogens to any product.
[g]
Wavelength used to photoactivate azido fluorogens.
[h]
Photobleaching quantum yield of amino fluorophore. Fluorescein in gelatin is 64×10−6.
[i]
In dichloromethane.
[j]
In acetonitrile.
[k]
In gelatin.
[l]
In PMMA.