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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Cancer Res. 2010 May 25;70(12):5096–5108. doi: 10.1158/0008-5472.CAN-09-4148

Figure 2. miR-520h Is Required for Invasive Phenotype.

Figure 2

A, transwell migration assay and matrigel invasion assay of MDA-MB-231/VC and 231/E1A transfected with miR-520h or control miRNA. Bars indicate the migration or invasion activity compared to that of 231/VC ± SD. Each assay was performed in three separate experiments. The asterisk indicates a significant difference (P<0.05) between miR-520h transfected and control miRNA transfected 231/VC cells. The significant difference (P<0.05) between miR-520h transfected and control miRNA transfected 231/E1A cells is indicated by the # symbol (P<0.05). B, left, the expression of miR-520h and miR-22 in MDA-MB-231/VC cells and HeLa cells after miR-520h inhibitor treatment for 72 hours was determined by agarose elephoresis (upper) and qRT-PCR (lower). Right, transwell migration assay and matrigel invasion assay of MDA-MB-231/VC and 231/E1A cells transfected with miR-520h inhibitor (Anti-miR-520h) or control inhibitor (Anti-miR-Control). Bars indicate the migration or invasion activity compared to that of 231/VC ± SD. Each assay was performed in three separate experiments. The asterisk indicates a significant difference (P<0.05) between miR-520h inhibitor transfected and control inhibitor transfected 231/VC cells. C, 231/VC, 231/VC-miR520h, 231/E1A, and 231/E1A-miR520h cells stably expressing luciferase were injected intravenously into immunodeficient SCID mice. left, bioluminescence imaging of lung colonizations were measured four weeks after transplantation. Bioluminescence was used to quantify lung colonization. Right, data shown are representative mice corresponding to marked data points. n=10; horizontal line represents median signal for each cohort. P-values were obtained by one-tailed Student’s t-test. D, incidence of lung colonization in mice that received tail-vein injection of miR-520h-overexpression or control 231/VC and 231/E1A cells.