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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Cancer Res. 2010 May 25;70(12):5096–5108. doi: 10.1158/0008-5472.CAN-09-4148

Figure 3. Induction of Twist is Critical for miR-520h Metastasis Promoting Function.

Figure 3

A, immunoblotting for E-cadherin, Twist, Slug in 231/VC, 231/E1A, and different stable clones of 231/E1A/miR-520h. B, whole cell extracts of MDA-MB-231 cells transfected with the miR-520h inhibitor (anti-miR-520h) or control inhibitor (anti-miR-control) were prepared 48 hours after transfection and analyzed by immunoblotting with Twist and tubulin antibodies. C, transwell migration assay (left) and matrigel invasion assay (right) of 231/VC, 231/E1A, and 231/E1A-miR520h overexpression cells stably transduced with Twist siRNA. Bars indicate the migration or invasion activity compared to that of 231/VC ± SD. The asterisk indicate a significant difference (P<0.05) between siTwist transduced and siControl tranduced 231/VC cells. The significant difference (P<0.05) between Twist knockdown and control knockdown 231/E1A-miR520h is indicated by the # symbol. D, 231/VC, 231/E1A, and 231/E1A-miR520h overexpression cells stably transduced with Twist siRNA and analyzed by immunoblotting with Twist and tubulin antibodies.