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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Cancer Res. 2010 May 25;70(12):5096–5108. doi: 10.1158/0008-5472.CAN-09-4148

Figure 5. Twist is Regulated by PP2A/C-mediated Signal Transduction Cascades.

Figure 5

A, left, immunoblotting of PP2A/C and Twist in 231/E1A and 231/E1A-miR-520h cells transiently transfected with PP2A/C expressing vector lacking the endogenous 3′UTR. Transwell migration assay (middle) and Matrigel invasion assay (right) of 231/VC, 231/E1A, and 231/E1A-miR-520h transiently transfected with PP2A/C expressing vector lacking the endogenous 3′UTR. Bars indicate the migration or invasion activity compared to that of 231/VC ± SD. The asterisk indicates a significant difference (P<0.05) between PP2A/C transfected and control vector transfected cells. B, immunoblotting of PP2A/C and Twist in 231/E1A and 231/E1A-miR-520h cells transiently transfected with PP2A/C siRNA. C, immunoblots showing the phosphorylation status of Akt and IKK. D, immunoblotting of Twist in 231/VC and 231/E1A cells transfected with a constitutive expression vector for myr-Akt (left) or IKK (right). E, immunoblotting of Twist and p-IKK in 231/VC and 231/E1A cells after the treatment of OA (10nM), PP2A phosphatase inhibitor at indicated time points.